Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activation.

ABSTRACT:

Aims

Sepsis-induced lung injury is associated with significant morbidity and mortality. Previously, we showed that heterozygous deletion of polymerase δ-interacting protein 2 (Poldip2) was protective against sepsis-induced lung injury. Since endothelial barrier disruption is thought to be the main mechanism of sepsis-induced lung injury, we sought to determine if the observed protection was specifically due to the effect of reduced endothelial Poldip2.

Methods and results

Endothelial-specific Poldip2 knock-out mice (EC-/-) and their wild-type littermates (EC+/+) were injected with saline or lipopolysaccharide (18 mg/kg) to model sepsis-induced lung injury. At 18 h post-injection mice, were euthanized and bronchoalveolar lavage (BAL) fluid and lung tissue were collected to as

SUBMITTER: Dolmatova EV 

PROVIDER: S-EPMC9612795 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature