The human milk proteome and allergy of mother and child: Exploring associations with protein abundances and protein network connectivity.

ABSTRACT:

Background

The human milk proteome comprises a vast number of proteins with immunomodulatory functions, but it is not clear how this relates to allergy of the mother or allergy development in the breastfed infant. This study aimed to explore the relation between the human milk proteome and allergy of both mother and child.

Methods

Proteins were analyzed in milk samples from a subset of 300 mother-child dyads from the Canadian CHILD Cohort Study, selected based on maternal and child allergy phenotypes. For this selection, the definition of "allergy" included food allergy, eczema, allergic rhinitis, and asthma. Proteins were analyzed with non-targeted shotgun proteomics using filter-aided sample preparation (FASP) and nanoLC-Orbitrap-MS/MS. Protein abundances, based on label-f

SUBMITTER: Dekker PM 

PROVIDER: S-EPMC9613325 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature