Inhibition of autophagy with chloroquine dysregulates mitochondrial quality control and energetics in adipocytes.

ABSTRACT: Autophagy is a complex degradation pathway through which damaged or dysfunctional proteins and organelles are removed. Its pharmacological modulators have been extensively used in a wide range of basic research and preclinical studies. However, the effects of these inhibitors on metabolism, in addition to autophagy inhibition, are not fully elucidated. Chloroquine is a clinically relevant compound that inhibits autophagy by preventing the fusion of autophagosomes with lysosomes. In this study, we aimed to examine the effect of chloroquine on mitochondrial quality control and respiratory function by utilizing 3T3-L1 mouse adipocytes treated with chloroquine at various time points. We found that chloroquine could disturb genes related to mitochondrial fission, biogenesis, and mitophagy, lead

SUBMITTER: Javaid HMA 

PROVIDER: S-EPMC9613452 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature