Integrated host-microbe plasma metagenomics for sepsis diagnosis in a prospective cohort of critically ill adults.

ABSTRACT: We carried out integrated host and pathogen metagenomic RNA and DNA next generation sequencing (mNGS) of whole blood (n = 221) and plasma (n = 138) from critically ill patients following hospital admission. We assigned patients into sepsis groups on the basis of clinical and microbiological criteria. From whole-blood gene expression data, we distinguished patients with sepsis from patients with non-infectious systemic inflammatory conditions using a trained bagged support vector machine (bSVM) classifier (area under the receiver operating characteristic curve (AUC) = 0.81 in the training set; AUC = 0.82 in a held-out validation set). Plasma RNA also yielded a transcriptional signature of sepsis with several genes previously reported as sepsis biomarkers, and a bSVM sepsis diagnostic classi

SUBMITTER: Kalantar KL 

PROVIDER: S-EPMC9613463 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature