Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS.

ABSTRACT: The measure of hair cortisol concentration (HCC) is becoming an emerging approach to monitor mid-/long-term stress in animals, so it is more and more important to develop accurate and reliable methods. In the light of this, the aim of the present study was to compare mane HCCs of 47 horses with different managements, by means of an immunoassay (ELISA) and liquid chromatography coupled to hybrid high-resolution mass spectrometry (LC-HRMS/MS). After the washing step, the ground hair was extracted with methanol. The extract was evaporated and redissolved in two different aqueous solutions, depending on the detection technique. The methods were validated according to EMA guideline for bioanalytical method validation, in the range 2-50 pg mg^-1 (ELISA) and 1-100 pg mg^-1 (LC

SUBMITTER: Saluti G 

PROVIDER: S-EPMC9613578 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature