Three-dimensional characterization of developing and adult ocular vasculature in mice using in toto clearing.

ABSTRACT: The ocular vasculature is critically involved in many blinding diseases and is also a popular research model for the exploration of developmental and pathological angiogenesis. The development of ocular vessels is a complex, finely orchestrated sequence of events, involving spatial and temporal coordination of hyaloid, choroidal and retinal networks. Comprehensive studies of the tridimensional dynamics of microvascular remodeling are limited by the fact that preserving the spatial disposition of ocular vascular networks is cumbersome using classical histological procedures. Here, we demonstrate that light-sheet fluorescence microscopy (LFSM) of cleared mouse eyes followed by extensive virtual dissection offers a solution to this problem. To the best of our knowledge, this is the first 3D q

SUBMITTER: Darche M 

PROVIDER: S-EPMC9613908 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature