Profiling Yeast Deletion Strains Using Sample Multiplexing and Network-Based Analyses.

ABSTRACT: The yeast, Saccharomyces cerevisiae, is a widely used model system for investigating conserved biological functions and pathways. Advancements in sample multiplexing have facilitated the study of the yeast proteome, yet many yeast proteins remain uncharacterized or only partially characterized. Yeast deletion strain collections are powerful resources for yeast proteome studies, uncovering the effects of gene function, genetic interactions, and cellular stresses. As complex biological systems cannot be understood by simply analyzing the individual components, a systems approach is often required in which a protein is represented as a component of large, interacting networks. Here, we evaluate the current state of yeast proteome analysis using isobaric tag-based sample multiplexing (T

SUBMITTER: Liu X 

PROVIDER: S-EPMC9614305 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature