Allogeneic and xenogeneic lymphoid reconstitution in a RAG2 ^-/- IL2RG ^y/- severe combined immunodeficient pig: A preclinical model for intrauterine hematopoietic transplantation.

ABSTRACT: Mice with severe combined immunodeficiency are commonly used as hosts of human cells. Size, longevity, and physiology, however, limit the extent to which immunodeficient mice can model human systems. To address these limitations, we generated RAG2 ^-/- IL2RG ^y/- immunodeficient pigs and demonstrate successful engraftment of SLA mismatched allogeneic D42 fetal liver cells, tagged with pH2B-eGFP, and human CD34⁺ hematopoietic stem cells after in utero cell transplantation. Following intrauterine injection at day 42-45 of gestation, fetuses were allowed to gestate to term and analyzed postnatally for the presence of pig (allogeneic) and human (xenogeneic) B cells, T-cells and NK cells in peripheral blood and other lymphoid tis

SUBMITTER: Sper RB 

PROVIDER: S-EPMC9614384 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature