Cell-cell contacts via N-cadherin induce a regulatory renin secretory phenotype in As4.1 cells.

ABSTRACT: The lack of a clonal renin-secreting cell line has greatly hindered the investigation of the regulatory mechanisms of renin secretion at the cellular, biochemical, and molecular levels. In the present study, we investigated whether it was possible to induce phenotypic switching of the renin-expressing clonal cell line As4.1 from constitutive inactive renin secretion to regulated active renin secretion. When grown to postconfluence for at least two days in media containing fetal bovine serum or insulin-like growth factor-1, the formation of cell-cell contacts via N-cadherin triggered downstream cellular signaling cascades and activated smooth muscle-specific genes, culminating in phenotypic switching to a regulated active renin secretion phenotype, including responding to the key sti

SUBMITTER: Chang JW 

PROVIDER: S-EPMC9614399 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature