Dynamic in Situ Confinement Triggers Ligand-Free Neuropeptide Receptor Signaling.

ABSTRACT: Membrane receptor clustering is fundamental to cell-cell communication; however, the physiological function of receptor clustering in cell signaling remains enigmatic. Here, we developed a dynamic platform to induce cluster formation of neuropeptide Y₂ hormone receptors (Y₂R) in situ by a chelator nanotool. The multivalent interaction enabled a dynamic exchange of histidine-tagged Y₂R within the clusters. Fast Y₂R enrichment in clustered areas triggered ligand-independent signaling as determined by an increase in cytosolic calcium and cell migration. Notably, the calcium and motility response to ligand-induced activation was amplified in preclustered cells, suggesting a key role of receptor clustering in sensitizing the dose response to lo

SUBMITTER: Sanchez MF 

PROVIDER: S-EPMC9614963 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature