Phenotypic selection during laboratory evolution of yeast populations leads to a genome-wide sustainable chromatin compaction shift.

ABSTRACT: In a previous study, we have shown how microbial evolution has resulted in a persistent reduction in expression after repeatedly selecting for the lowest P_GAL1-YFP-expressing cells. Applying the ATAC-seq assay on samples collected from this 28-day evolution experiment, here we show how genome-wide chromatin compaction changes during evolution under selection pressure. We found that the chromatin compaction was altered not only on GAL network genes directly impacted by the selection pressure, showing an example of selection-induced non-genetic memory, but also at the whole-genome level. The GAL network genes experienced chromatin compaction accompanying the reduction in P_GAL1-YFP reporter expression. Strikingly, the fraction of global genes with differentially compacte

SUBMITTER: Moreno DF 

PROVIDER: S-EPMC9615041 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature