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ScTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells.

ABSTRACT: Single-cell DNA methylation profiling currently suffers from excessive noise and/or limited cellular throughput. We developed scTAM-seq, a targeted bisulfite-free method for profiling up to 650 CpGs in up to 10,000 cells per experiment, with a dropout rate as low as 7%. We demonstrate that scTAM-seq can resolve DNA methylation dynamics across B-cell differentiation in blood and bone marrow, identifying intermediate differentiation states that were previously masked. scTAM-seq additionally queries surface-protein expression, thus enabling integration of single-cell DNA methylation information with cell atlas data. In summary, scTAM-seq is a high-throughput, high-confidence method for analyzing DNA methylation at single-CpG resolution across thousands of single cells.

SUBMITTER: Bianchi A

PROVIDER: S-EPMC9615163 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells.

Bianchi Agostina A Scherer Michael M Zaurin Roser R Quililan Kimberly K Velten Lars L Beekman Renée R

Genome biology 20221028 1

Single-cell DNA methylation profiling currently suffers from excessive noise and/or limited cellular throughput. We developed scTAM-seq, a targeted bisulfite-free method for profiling up to 650 CpGs in up to 10,000 cells per experiment, with a dropout rate as low as 7%. We demonstrate that scTAM-seq can resolve DNA methylation dynamics across B-cell differentiation in blood and bone marrow, identifying intermediate differentiation states that were previously masked. scTAM-seq additionally querie ...[more]

PMID: 36307828

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