Gene cloning and molecular characterization of a thermostable chitosanase from Bacillus cereus TY24.

ABSTRACT:

Background

An important conceptual advance in health and the environment has been recognized that enzymes play a key role in the green processing industries. Of particular interest, chitosanase is beneficial for recycling the chitosan resource and producing chitosan oligosaccharides. Also, chitosan gene expression and molecular characterization will promote understanding of the biological function of bacterial chitosanase as well as explore chitosanase for utilizing chitosan resources.

Results

A chitosanase-producing bacterium TY24 was isolated and identified as Bacillus cereus. Moreover, the chitosanase gene was cloned and expressed in Escherichia coli. Sequence analysis reveals that the recombinant chitosanase (CHOE) belongs to the glycoside hydrolases 8 family. The purifi

SUBMITTER: Zhang RX 

PROVIDER: S-EPMC9615241 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature