Characterization of a multipurpose NS3 surface patch coordinating HCV replicase assembly and virion morphogenesis.

ABSTRACT: The hepatitis C virus (HCV) life cycle is highly regulated and characterized by a step-wise succession of interactions between viral and host cell proteins resulting in the assembly of macromolecular complexes, which catalyse genome replication and/or virus production. Non-structural (NS) protein 3, comprising a protease and a helicase domain, is involved in orchestrating these processes by undergoing protein interactions in a temporal fashion. Recently, we identified a multifunctional NS3 protease surface patch promoting pivotal protein-protein interactions required for early steps of the HCV life cycle, including NS3-mediated NS2 protease activation and interactions required for replicase assembly. In this work, we extend this knowledge by identifying further NS3 surface determinants imp

SUBMITTER: Isken O 

PROVIDER: S-EPMC9616216 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature