TLR2 and TLR4 activity in monocytes and macrophages after exposure to amoxicillin, ciprofloxacin, doxycycline and erythromycin.

ABSTRACT:

Background

Antibiotics are used to treat bacterial infections but also impact immunity. This is usually attributed to antibiotic-induced dysbiosis of the microbiota, but antibiotics may have a direct effect on immune cells and immunity-associated receptors, such as Toll-like receptors (TLRs).

Objectives

To investigate whether antibiotics alter TLR2/1, TLR2/6 and TLR4 activity in immune cells.

Methods

We evaluated the effects of amoxicillin, ciprofloxacin, doxycycline and erythromycin on TLR2/1-, TLR2/6- and TLR4-induced NF-κB activation in THP1-XBlue™-MD2-CD14 cells. Furthermore, we studied TNF-α and IL-6 levels in THP-1-derived macrophages after exposure to these antibiotics and TLR ligands.

Results

Amoxicillin had no effect on any of the TLRs studied. However

SUBMITTER: Silva Lagos L 

PROVIDER: S-EPMC9616545 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature