Establishment of centromere identity is dependent on nuclear spatial organization.

ABSTRACT: The establishment of centromere-specific CENP-A chromatin is influenced by epigenetic and genetic processes. Central domain sequences from fission yeast centromeres are preferred substrates for CENP-A^Cnp1 incorporation, but their use is context dependent, requiring adjacent heterochromatin. CENP-A^Cnp1 overexpression bypasses heterochromatin dependency, suggesting that heterochromatin ensures exposure to conditions or locations permissive for CENP-A^Cnp1 assembly. Centromeres cluster around spindle-pole bodies (SPBs). We show that heterochromatin-bearing minichromosomes localize close to SPBs, consistent with this location promoting CENP-A^Cnp1 incorporation. We demonstrate that heterochromatin-independent de novo CENP-A^Cnp1 chromatin ass

SUBMITTER: Wu W 

PROVIDER: S-EPMC9616734 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature