Genome-scale modeling of Chinese hamster ovary cells by hybrid semi-parametric flux balance analysis.

ABSTRACT: Flux balance analysis (FBA) is currently the standard method to compute metabolic fluxes in genome-scale networks. Several FBA extensions employing diverse objective functions and/or constraints have been published. Here we propose a hybrid semi-parametric FBA extension that combines mechanistic-level constraints (parametric) with empirical constraints (non-parametric) in the same linear program. A CHO dataset with 27 measured exchange fluxes obtained from 21 reactor experiments served to evaluate the method. The mechanistic constraints were deduced from a reduced CHO-K1 genome-scale network with 686 metabolites, 788 reactions and 210 degrees of freedom. The non-parametric constraints were obtained by principal component analysis of the flux dataset. The two types of constraints were integ

SUBMITTER: Ramos JRC 

PROVIDER: S-EPMC9616788 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature