A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing.

ABSTRACT: The experimental need to engineer the genome both in time and space, has led to the development of several photoactivatable Cre recombinase systems. However, the combination of inefficient and non-intentional background recombination has prevented thus far the wide application of these systems in biological and biomedical research. Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre). Following extensive characterization in cancer cell and organoid systems, we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy. These experiments illustrate how newly formed HrasV12 mutan

SUBMITTER: Vizoso M 

PROVIDER: S-EPMC9616875 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature