Immune phenotype of the CD4⁺ T cells in the aged lymphoid organs and lacrimal glands.

ABSTRACT: Aging is associated with a massive infiltration of T lymphocytes in the lacrimal gland. Here, we aimed to characterize the immune phenotype of aged CD4⁺ T cells in this tissue as compared with lymphoid organs. To perform this, we sorted regulatory T cells (Tregs, CD4⁺CD25⁺GITR⁺) and non-Tregs (CD4⁺CD25^negGITR^neg) in lymphoid organs from female C57BL/6J mice and subjected these cells to an immunology NanoString® panel. These results were confirmed by flow cytometry, live imaging, and tissue immunostaining in the lacrimal gland. Importantly, effector T helper 1 (Th1) genes were highly upregulated on aged Tregs, including the master regulator Tbx21. Among the non-Tregs, we also found a significant increase in the lev

SUBMITTER: Trujillo-Vargas CM 

PROVIDER: S-EPMC9617003 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature