Production of CFTR Mutant Gene Model by Homologous Recombination System.

ABSTRACT:

Objective

The most common mutation in cystic fibrosis (CF), (ΔF508-CFTR), results in impaired protein maturation, folding and transportation to the surface of the cell. As a consequence of impaired protein maturation and/or transport from the extracellular matrix to the cell, different systems are influenced, including gastrointestinal system and glandular system, reproductive system and respiratory systems. CF models are essential tools to provide further knowledge of CF pathophysiology. With this aim, we designed a transgenic CF model based on the homologous recombination (HR) system.

Materials and methods

In this experimental study, a specifically designed construct containing the CFTR gene with F508del was cloned into a PTZ57R cloning vector and then the construct

SUBMITTER: Rezaee H 

PROVIDER: S-EPMC9617022 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature