Single-cell profiling reveals distinct adaptive immune hallmarks in MDA5+ dermatomyositis with therapeutic implications.

ABSTRACT: Anti-melanoma differentiation-associated gene 5-positive dermatomyositis (MDA5⁺ DM) is an autoimmune condition associated with rapidly progressive interstitial lung disease and high mortality. The aetiology and pathogenesis of MDA5⁺ DM are still largely unknown. Here we describe the immune signatures of MDA5⁺ DM via single-cell RNA sequencing, flow cytometry and multiplex immunohistochemistry in peripheral B and T cells and in affected lung tissue samples from one patient. We find strong peripheral antibody-secreting cell and CD8⁺ T cell responses as cellular immune hallmarks, and over-stimulated type I interferon signaling and associated metabolic reprogramming as molecular immune signature in MDA5⁺ DM. High frequency of circulating I

SUBMITTER: Ye Y 

PROVIDER: S-EPMC9617246 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature