Microaerobic metabolism of lactate and propionate enhances vitamin B₁₂ production in Propionibacterium freudenreichii.

ABSTRACT:

Background

Propionibacterium freudenreichii is used in biotechnological applications to produce vitamin B₁₂. Although cultured mainly in anaerobic conditions, microaerobic conditions can greatly enhance biomass formation in P. freudenreichii. Since B₁₂ yields may be coupled to biomass formation, microaerobic conditions show great potential for increasing B₁₂ yields in P. freudenreichii.

Results

Here we show biomass formation increases 2.7 times for P. freudenreichii grown in microaerobic conditions on lactate versus anaerobic conditions (1.87 g/L vs 0.70 g/L). Consumption of lactate in microaerobic conditions resulted first in production of pyruvate, propionate and acetate. When lactate was depleted, pyruvate and propionate were oxidised with

SUBMITTER: Dank A 

PROVIDER: S-EPMC9617374 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature