A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean.

ABSTRACT:

Background

Soybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic overexpression of proteins-of-interest, because soybean transformation is time-consuming and technically challenging. An attractive alternative is to administer transient gene silencing or overexpression with a plant virus-based vector. However, existing virus-induced gene silencing (VIGS) and/or overexpression vectors suitable for soybean have various drawbacks that hinder their widespread adoption.

Results

We describe the development of a new vector based on cowpea severe mosaic virus (CPSMV), a plus-strand RNA virus with its genome divided into two RNA segments, RNA1 and RNA2. This vector, designated FZ, incorporates a cloning site

SUBMITTER: Zaulda FA 

PROVIDER: S-EPMC9617382 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature