A high-throughput screening assay to identify inhibitory antibodies targeting alphavirus release.

ABSTRACT:

Background

Several studies have demonstrated neutralizing antibodies to be highly effective against alphavirus infection in animal models, both prophylactically and remedially. In most studies, neutralizing antibodies have been evaluated for their ability to block viral entry in vitro but recent evidence suggests that antibody inhibition through other mechanisms, including viral budding/release, significantly contributes to viral control in vivo for a number of alphaviruses.

Results

We describe a BSL-2, cell-based, high-throughput screening system that specifically screens for inhibitors of alphavirus egress using chikungunya virus (CHIKV) and Mayaro virus (MAYV) novel replication competent nano-luciferase (nLuc) reporter viruses. Screening of both polyclonal sera and memory

SUBMITTER: Ramjag A 

PROVIDER: S-EPMC9617529 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature