De novo transcriptome assembly using Illumina sequencing and development of EST-SSR markers in a monoecious herb Sagittaria trifolia Linn.

ABSTRACT:

Background

Sagittaria trifolia Linn. is a widespread macrophyte in Asia and southeast Europe and cultivated in parts of Asia. Although a few genomic studies have been conducted for S. trifolia var. sinensis, a crop breed, there is limited genomic information on the wild species of S. trifolia. Effective microsatellite markers are also lacking.

Objective

To assemble transcriptome sequence and develop effective EST-SSR markers for S. trifolia.

Methods

Here we developed microsatellite markers based on tri-, tetra-, penta-, and hexa-nucleotide repeat sequences by comparatively screening multiple transcriptome sequences of eleven individuals from ten natural populations of S. trifolia.

Results

A total of 107,022 unigenes wer

SUBMITTER: Tang H 

PROVIDER: S-EPMC9617548 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature