Long-read sequencing characterizes mitochondrial and plastid genome variants in Arabidopsis msh1 mutants.

ABSTRACT: The abundant repeats in plant mitochondrial genomes can cause rapid genome rearrangements and are also a major obstacle in short-read sequencing studies. Nuclear-encoded proteins such as MSH1 are known to suppress the generation of repeat-associated mitochondrial genome variants, but our understanding of these mechanisms has been constrained by the limitations of short-read technologies. Here, we used highly accurate long-read sequencing (PacBio HiFi) to characterize mitochondrial and plastid genome variants in Arabidopsis thaliana msh1 mutant individuals. The HiFi reads provided a global view of recombination dynamics with detailed quantification of parental and crossover recombination products for both large and small repeats. We found that recombination breakpoints were distributed rela

SUBMITTER: Zou Y 

PROVIDER: S-EPMC9617793 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature