Validation of donor fraction cell-free DNA with biopsy-proven cardiac allograft rejection in children and adults.

ABSTRACT:

Objectives

Donor-specific cell-free DNA shows promise as a noninvasive marker for allograft rejection, but as yet has not been validated in both adult and pediatric recipients. The study objective was to validate donor fraction cell-free DNA as a noninvasive test to assess for risk of acute cellular rejection and antibody-mediated rejection after heart transplantation in pediatric and adult recipients.

Methods

Pediatric and adult heart transplant recipients were enrolled from 7 participating sites and followed for 12 months or more with plasma samples collected immediately before all endomyocardial biopsies. Donor fraction cell-free DNA was extracted, and quantitative genotyping was performed. Blinded donor fraction cell-free DNA and clinical data were analyzed and compared

SUBMITTER: Richmond ME 

PROVIDER: S-EPMC9617812 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature