Structural insights into light-driven anion pumping in cyanobacteria.

ABSTRACT: Transmembrane ion transport is a key process in living cells. Active transport of ions is carried out by various ion transporters including microbial rhodopsins (MRs). MRs perform diverse functions such as active and passive ion transport, photo-sensing, and others. In particular, MRs can pump various monovalent ions like Na⁺, K⁺, Cl^-, I^-, NO₃^-. The only characterized MR proposed to pump sulfate in addition to halides belongs to the cyanobacterium Synechocystis sp. PCC 7509 and is named Synechocystis halorhodopsin (SyHR). The structural study of SyHR may help to understand what makes an MR pump divalent ions. Here we present the crystal structure of SyHR in the ground state, the structure of its sulfate-bound form as well as

SUBMITTER: Astashkin R 

PROVIDER: S-EPMC9617919 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature