Regulated Restructuring of Mucins During Secretory Granule Maturation In Vivo.

ABSTRACT: Mucins are large, highly glycosylated transmembrane and secreted proteins that line and protect epithelial surfaces. However, the details of mucin biosynthesis and packaging in vivo are largely unknown. Here, we demonstrate that multiple distinct mucins undergo intragranular restructuring during secretory granule maturation in vivo, forming unique structures that are spatially segregated within the same granule. We further identify temporally-regulated genes that influence mucin restructuring, including those controlling pH (Vha16-1), Ca²⁺ ions (fwe) and Cl^- ions (Clic and ClC-c). Finally, we show that altered mucin glycosylation influences the dimensions of these structures, thereby affecting secretory granule morphology. This study elucid

SUBMITTER: Syed ZA 

PROVIDER: S-EPMC9618048 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature