A genetically encoded system for oxygen generation in living cells.

ABSTRACT: Oxygen plays a key role in supporting life on our planet. It is particularly important in higher eukaryotes where it boosts bioenergetics as a thermodynamically favorable terminal electron acceptor and has important roles in cell signaling and development. Many human diseases stem from either insufficient or excessive oxygen. Despite its fundamental importance, we lack methods with which to manipulate the supply of oxygen with high spatiotemporal resolution in cells and in organisms. Here, we introduce a genetic system, SupplemeNtal Oxygen Released from ChLorite (SNORCL), for on-demand local generation of molecular oxygen in living cells, by harnessing prokaryotic chlorite O₂-lyase (Cld) enzymes that convert chlorite (ClO₂^-) into molecular oxygen (O_2</

SUBMITTER: Markhard AL 

PROVIDER: S-EPMC9618058 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature