Global profiling of arginine dimethylation in regulating protein phase separation by a steric effect-based chemical-enrichment method.

ABSTRACT: Protein arginine methylation plays an important role in regulating protein functions in different cellular processes, and its dysregulation may lead to a variety of human diseases. Recently, arginine methylation was found to be involved in modulating protein liquid-liquid phase separation (LLPS), which drives the formation of different membraneless organelles (MLOs). Here, we developed a steric effect-based chemical-enrichment method (SECEM) coupled with liquid chromatography-tandem mass spectrometry to analyze arginine dimethylation (DMA) at the proteome level. We revealed by SECEM that, in mammalian cells, the DMA sites occurring in the RG/RGG motifs are preferentially enriched within the proteins identified in different MLOs, especially stress granules (SGs). Notably, global decrease of

SUBMITTER: Wang Q 

PROVIDER: S-EPMC9618127 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature