Glioblastoma cell motility depends on enhanced oxidative stress coupled with mobilization of a sulfurtransferase.

ABSTRACT: Cell motility is critical for tumor malignancy. Metabolism being an obligatory step in shaping cell behavior, we looked for metabolic weaknesses shared by motile cells across the diverse genetic contexts of patients' glioblastoma. Computational analyses of single-cell transcriptomes from thirty patients' tumors isolated cells with high motile potential and highlighted their metabolic specificities. These cells were characterized by enhanced mitochondrial load and oxidative stress coupled with mobilization of the cysteine metabolism enzyme 3-Mercaptopyruvate sulfurtransferase (MPST). Functional assays with patients' tumor-derived cells and -tissue organoids, and genetic and pharmacological manipulations confirmed that the cells depend on enhanced ROS production and MPST activity for their m

SUBMITTER: Saurty-Seerunghen MS 

PROVIDER: S-EPMC9618559 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature