Honing-in antigen-specific cells during antibody discovery: a user-friendly process to mine a deeper repertoire

ABSTRACT: Immunization based antibody discovery is plagued by the paucity of antigen-specific B cells. Identifying these cells is akin to finding needle in a haystack. Current and emerging technologies while effective, are limited in terms of capturing the antigen-specific repertoire. We report on the bulk purification of antigen-specific B-cells and the benefits it offers to various antibody discovery platforms. Using five different antigens, we show hit rates of 51–88%, compared to about 5% with conventional methods. We also show that this purification is highly efficient with loss of only about 2% antigen specific cells. Furthermore, we compared clones in which cognate chains are preserved with those from display libraries in which chains either from total B cells (TBC) or antigen-specific B cell

SUBMITTER: Mahendra A 

PROVIDER: S-EPMC9618561 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature