HAT-field: a cheap, robust and quantitative point-of-care serological test for covid-19

ABSTRACT: Abstract The HAT-field protocol described here is an optimization of the recently published HAT hemagglutination test, for the detection of antibodies directed against the receptor binding domain (RBD) of the SARS-Cov-2 virus (Townsend et al. 2021). HAT and HAT-field are both based on hemagglutination triggered by a single reagent, the IH4-RBD recombinant protein. A sample of IH4-RBD sufficient for several thousand tests or a plasmid encoding IH4-RBD can be obtained from the authors of our first paper (Townsend et al. 2021). Using titration of IH4-RBD, HAT-field now allows a quantitative assessment of antibody levels in a single step, using a few microliters of whole blood, such as can be obtained by finger prick, and requires only very simple disposable equipment. Because it is based on a

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PROVIDER: S-EPMC9620368 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature