Temporal brain microRNA expression changes in a mouse model of neonatal hypoxic-ischemic injury.

ABSTRACT:

Background

Neonatal hypoxic-ischemic brain injury (HIBI) results in significant morbidity and mortality despite current standard therapies. MicroRNAs (miRNAs) are a promising therapeutic target; however, there is a paucity of data on endogenous miRNA expression of the brain after HIBI during the primary therapeutic window (6-72 h after injury).

Methods

Postnatal day 9 mouse pups underwent unilateral carotid ligation+hypoxia (HIBI), sham surgery+hypoxia, or sham surgery+normoxia (controls). miRNA sequencing was performed on the ipsilateral brain of each of the three groups plus the contralateral HIBI brain at 24 and 72 h after injury. Findings were validated in eight key miRNAs by quantitative polymerase chain reaction.

Results

Hypoxia resulted in significant different

SUBMITTER: Peeples ES 

PROVIDER: S-EPMC9620396 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature