DNA-Based Optical Quantification of Ion Transport across Giant Vesicles.

ABSTRACT: Accurate measurements of ion permeability through cellular membranes remains challenging due to the lack of suitable ion-selective probes. Here we use giant unilamellar vesicles (GUVs) as membrane models for the direct visualization of mass translocation at the single-vesicle level. Ion transport is indicated with a fluorescently adjustable DNA-based sensor that accurately detects sub-millimolar variations in K⁺ concentration. In combination with microfluidics, we employed our DNA-based K⁺ sensor for extraction of the permeation coefficient of potassium ions. We measured K⁺ permeability coefficients at least 1 order of magnitude larger than previously reported values from bulk experiments and show that permeation rates across the lipid bilayer increase in t

SUBMITTER: Fletcher M 

PROVIDER: S-EPMC9620405 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature