Bottom-up proteomic analysis of human adult cardiac tissue and isolated cardiomyocytes.

ABSTRACT: The heart is composed of multiple cell types, each with a specific function. Cell-type-specific approaches are necessary for defining the intricate molecular mechanisms underlying cardiac development, homeostasis, and pathology. While single-cell RNA-seq studies are beginning to define the chamber-specific cellular composition of the heart, our views of the proteome are more limited because most proteomics studies have utilized homogenized human cardiac tissue. To promote future cell-type specific analyses of the human heart, we describe the first method for cardiomyocyte isolation from cryopreserved human cardiac tissue followed by flow cytometry for purity assessment. We also describe a facile method for preparing isolated cardiomyocytes and whole cardiac tissue homogenate for bottom-up

SUBMITTER: Wojtkiewicz M 

PROVIDER: S-EPMC9620472 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature