Insights into the structure and function of the histidine kinase ComP from Bacillus amyloliquefaciens based on molecular modeling.

ABSTRACT: The ComPA two-component signal transduction system (TCS) is essential in Bacillus spp. However, the molecular mechanism of the histidine kinase ComP remains unclear. Here, we predicted the structure of ComP from Bacillus amyloliquefaciens Q-426 (BaComP) using an artificial intelligence approach, analyzed the structural characteristics based on the molecular docking results and compared homologous proteins, and then investigated the biochemical properties of BaComP. We obtained a truncated ComPS protein with high purity and correct folding in solution based on the predicted structures. The expression and purification of BaComP proteins suggested that the subdomains in the cytoplasmic region influenced the expression and stability of the recombinant proteins. ComPS is a bifunctional enzyme t

SUBMITTER: Wang L 

PROVIDER: S-EPMC9620489 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature