Systematic replication enables normalization of high-throughput imaging assays.

ABSTRACT:

Motivation

High-throughput fluorescent microscopy is a popular class of techniques for studying tissues and cells through automated imaging and feature extraction of hundreds to thousands of samples. Like other high-throughput assays, these approaches can suffer from unwanted noise and technical artifacts that obscure the biological signal. In this work, we consider how an experimental design incorporating multiple levels of replication enables the removal of technical artifacts from such image-based platforms.

Results

We develop a general approach to remove technical artifacts from high-throughput image data that leverages an experimental design with multiple levels of replication. To illustrate the methods, we consider microenvironment microarrays (MEMAs), a high-throughpu

SUBMITTER: Hunt GJ 

PROVIDER: S-EPMC9620822 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature