M⁶A RNA demethylase FTO promotes the growth, migration and invasion of pancreatic cancer cells through inhibiting TFPI-2.

ABSTRACT: Pancreatic cancer (PC) is one of the most fatal cancers with a very poor prognosis. Here, we found that N⁶-methyladenosine (m⁶A) RNA demethylase fat mass and obesity-related protein (FTO) promote the growth, migration and invasion of PC. FTO expression level is increased in human PC and is associated with poor prognosis of PC patients. Knockdown of FTO increases m⁶A methylation of TFPI-2 mRNA in PC cells, thereby increasing mRNA stability via the m⁶A reader YTHDF1, resulting in up-regulation of TFPI-2 expression, and inhibits PC proliferation, colony formation, sphere formation, migration and invasion in vitro, as well as tumour growth in vivo. Rescue assay further confirms that FTO facilitates cancer progression by reducing the exp

SUBMITTER: Wang W 

PROVIDER: S-EPMC9621031 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature