Autophagic flux restoration enhances the antitumor efficacy of tumor infiltrating lymphocytes.

ABSTRACT:

Background

Although adoptive cell therapy with tumor infiltrating lymphocytes (TILs) has mediated effective antitumor responses in several cancers, dysfunction and exhaustion of TILs significantly impair the therapeutic effect of TILs. Thus, it is essential to elucidate the exhausted characteristics of TILs and improve the antitumor effect of TILs by reversing their exhaustion. Here, we focused on the influence of autophagy on TILs in terms of T-cell activation, proliferation, and differentiation in vitro and in vivo.

Methods

We first evaluated autophagy level of TILs and influence of spermidine treatment on autophagy levels of TILs. Furthermore, we assessed the proliferative potential, phenotypical characteristics, T cell receptor (TCR) repertoire and antitumor activity of

SUBMITTER: Zhang C 

PROVIDER: S-EPMC9621197 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature