Altered microvasculature in pancreatic islets from subjects with type 1 diabetes.

ABSTRACT:

Aims

The transcriptome of different dissociated pancreatic islet cells has been described in enzymatically isolated islets in both health and disease. However, the isolation, culturing, and dissociation procedures likely affect the transcriptome profiles, distorting the biological conclusions. The aim of the current study was to characterize the cells of the islets of Langerhans from subjects with and without type 1 diabetes in a way that reflects the in vivo situation to the highest possible extent.

Methods

Islets were excised using laser capture microdissection directly from frozen pancreatic tissue sections obtained from organ donors with (n = 7) and without (n = 8) type 1 diabetes. Transcriptome analysis of excised samples was performed using AmpliSeq. Consecutive pancre

SUBMITTER: Granlund L 

PROVIDER: S-EPMC9621430 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature