Integrated experimental-computational analysis of a HepaRG liver-islet microphysiological system for human-centric diabetes research.

ABSTRACT: Microphysiological systems (MPS) are powerful tools for emulating human physiology and replicating disease progression in vitro. MPS could be better predictors of human outcome than current animal models, but mechanistic interpretation and in vivo extrapolation of the experimental results remain significant challenges. Here, we address these challenges using an integrated experimental-computational approach. This approach allows for in silico representation and predictions of glucose metabolism in a previously reported MPS with two organ compartments (liver and pancreas) connected in a closed loop with circulating medium. We developed a computational model describing glucose metabolism over 15 days of culture in the MPS. The model was calibrated on an experiment-specific basis using data f

SUBMITTER: Casas B 

PROVIDER: S-EPMC9621595 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature