Role of reactive oxygen species in high concentration glucose-induced growth inhibition of human peritoneal mesothelial cells.

ABSTRACT:

Background

To investigate the effects and mechanism of high concentration glucose (HG), exogenous hydrogen peroxide (H₂O₂), and antioxidants on the cell growth (cell proliferation) of human peritoneal mesothelial cells (HPMCs).

Methods

All tests were conducted on cultured HPMCs (HMrSV5) in vitro. Various concentrations of glucose (0.1%, 1.35%, and 3.86%), H₂O₂ (0.5 and 0.1 mmol/L), and antioxidants (pyruvate and catalase) were used in cell culture. Moreover, in order to study the interaction between these factors, HG and H₂O₂, HG and antioxidants, HG, H₂O₂, and antioxidants, were used respectively. After 12-24 h, phase-contrast microscopy was used to examine the morphological chang

SUBMITTER: Zhu N 

PROVIDER: S-EPMC9622488 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature