Increased intracellular Cl^- concentration mediates neutrophil extracellular traps formation in atherosclerotic cardiovascular diseases.

ABSTRACT: Neutrophil extracellular traps (NETs) play crucial roles in atherosclerotic cardiovascular diseases such as acute coronary syndrome (ACS). Our preliminary study shows that oxidized low-density lipoprotein (oxLDL)-induced NET formation is accompanied by an elevated intracellular Cl^- concentration ([Cl^-]_i) and reduced cystic fibrosis transmembrane conductance regulator (CFTR) expression in freshly isolated human blood neutrophils. Herein we investigated whether and how [Cl^-]_i regulated NET formation in vitro and in vivo. We showed that neutrophil [Cl^-]_i and NET levels were increased in global CFTR null (Cftr^-/-) mice in the resting state, which was mimicked by intravenous injection of the CFTR inhibitor,

SUBMITTER: Han H 

PROVIDER: S-EPMC9622838 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature