Hypoxia-driven metabolic reprogramming of adipocytes fuels cancer cell proliferation.

ABSTRACT:

Objective

Obesity increases the risk of certain cancers, especially tumours that reside close to adipose tissue (breast and ovarian metastasis in the omentum). The obesogenic and tumour micro-environment share a common pathogenic feature, oxygen deprivation (hypoxia). Here we test how hypoxia changes the metabolome of adipocytes to assist cancer cell growth.

Methods

Human and mouse breast and ovarian cancer cell lines were co-cultured with human and mouse adipocytes respectively under normoxia or hypoxia. Proliferation and lipid uptake in cancer cells were measured by commercial assays. Metabolite changes under normoxia or hypoxia were measured in the media of human adipocytes by targeted LC/MS.

Results

Hypoxic cancer-conditioned media increased lipolysis in both huma

SUBMITTER: Aird R 

PROVIDER: S-EPMC9623062 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature