Identification and functional analysis of m⁶A in the mammary gland tissues of dairy goats at the early and peak lactation stages.

ABSTRACT: N6-methyladenosine (m⁶A) is the most common reversible epigenetic RNA modification in the mRNA of all higher eukaryotic organisms and plays an important role in the regulation of gene expression and cell function. In this study, m⁶A-modified methylated RNA immunoprecipitation sequencing (MeRIP-seq) and transcriptome sequencing (RNA-seq) were used to identify the key genes with m⁶A modification during mammary gland development and lactation in dairy goats. The results showed that m⁶A methylation occurred at 3,927 loci, which were significantly enriched in the 3' untranslated region (3'UTR) and the termination codon region. In the early stage and peak stage of lactation, m⁶A methylation occurred extensively in mammary tissues, and a tota

SUBMITTER: Wang S 

PROVIDER: S-EPMC9623301 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature