Single-cell assessment of the modulation of macrophage activation by ex vivo intervertebral discs using impedance cytometry.

ABSTRACT: Measurement of macrophage activation and its modulation for immune regulation is of great interest to arrest inflammatory responses associated with degeneration of intervertebral discs that cause chronic back pain, and with transplants that face immune rejection. Due to the phenotypic plasticity of macrophages that serve multiple immune functions, the net disease outcome is determined by a balance of subpopulations with competing functions, highlighting the need for single-cell methods to quantify heterogeneity in their activation phenotypes. However, since macrophage activation can follow several signaling pathways, cytometry after fluorescent staining of markers with antibodies does not often provide dose-dependent information on activation dynamics. We present high throughput single-cel

SUBMITTER: Salahi A 

PROVIDER: S-EPMC9623412 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature