Thioredoxin-1 regulates self-renewal and differentiation of murine hematopoietic stem cells through p53 tumor suppressor.

ABSTRACT:

Background

Thioredoxin-1 (TXN1) is one of the major cellular antioxidants in mammals and is involved in a wide range of physiological cellular responses. However, little is known about the roles and the underlying molecular mechanisms of TXN1 in the regulation of hematopoietic stem/progenitor cells (HSPCs).

Methods

TXN1 conditional knockout mice (ROSA-CreER-TXN1^fl/fl) and TXN1^fl/fl control mice were used. The mice were treated with tamoxifen and the number and biological functions of HSPCs were measured by flow cytometry, PCR and western blot. Limiting dilution competitive transplantation with sorted HSCs and serial transplantations were performed to assess the effects of TXN1 knockout on HSC self-renewal and long-term reconstitutional capacity. RNA seq

SUBMITTER: Jabbar S 

PROVIDER: S-EPMC9624023 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature