Analysis of different adipose depot gene expression in cachectic patients with gastric cancer.

ABSTRACT:

Purpose

This study aimed to identify the differentially expressed genes (DEGs) that contributed to the different amount of fat loss between subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) among cachectic patients.

Methods

RNA sequencing was performed and bioinformatic tools were utilized to analyze the biological functions and construct regulation networks of DEGs. We presumed that iroquois homeobox 1 (IRX1) to be a hub gene and analyzed its clinical significance. Mouse model of cancer cachexia was established and differences between SAT and VAT were compared. The function of IRX1 on lipid metabolism was clarified by Oil Red O staining, qRT-PCR, and Western blotting in adipocytes.

Results

A total of 455 DEGs were screened between SAT and VAT in cac

SUBMITTER: Han J 

PROVIDER: S-EPMC9624057 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature